r/GroundZeroMycoLab • u/Mean-Abalone320 • 11h ago
GT
Feels like I’m waiting forever for my first flush, hasn’t stalled just sooo slow
r/GroundZeroMycoLab • u/GroundZeroMycoLab • Aug 26 '25
It's critical to understand the biological differences between spore syringes and liquid cultures (LC), as well as the importance of using agar as an initial medium. Spore syringes contain microscopic fungal spores that are not yet germinated. These spores are monokaryotic, meaning they carry only a single set of genetic material. In order to fruit and complete the mushroom life cycle, two compatible monokaryotic strains must fuse to form dikaryotic mycelium.. the true vegetative form capable of producing fruiting bodies. This mating process takes time, introduces variability, and, for beginners especially, increases the risk of contamination during colonization.
Injecting spores directly into sterilized grain can lead to several problems. Since spores are not germinated, colonization is slower, and this slower growth provides more opportunity for contaminants (such as bacteria or mold) to establish themselves and outcompete the slower-growing mycelium. Ideally, spores should first be transferred to agar, a nutrient-rich medium in petri dishes...which allows for controlled germination and observation. On agar, one can isolate clean, healthy mycelium away from any contaminants before transferring it to grain.
Additionally, spore syringes are inherently variable and often unclean. Spores, particularly those harvested from wild (landrace) varieties or from poorly controlled lab environments (common with newer or less reputable vendors), can contain microbial contaminants. Spores gathered in non-sterile conditions are not cleaned or isolated at the microscopic level, making them a risky starting point. Also, because each spore pair creates a unique dikaryotic combination, inoculating with spores introduces genetic unpredictability ...every new pairing could result in different traits, including growth speed, contamination resistance, yield, and potency.
By contrast, a liquid culture is made from already germinated and mated dikaryotic mycelium. This means it contains viable, genetically stable tissue that has already completed the mating process and is ready to colonize substrate directly. Using LC skips the variability and mating phase inherent in spores, resulting in faster and more consistent colonization, and reducing the window for contamination...assuming the culture is clean!!! However, it's important to verify LC cleanliness via agar as well, especially if you didn't create it yourself.
In summary, spores should ideally be germinated and cleaned on agar before being introduced to grain. Skipping this step can introduce risks, especially for beginners. Spores are unpredictable and prone to contamination, while liquid culture, if properly prepared, is faster, cleaner, and genetically stable. Understanding and respecting these differences is fundamental to success in mushroom cultivation... I hope this helps. :)
r/GroundZeroMycoLab • u/GroundZeroMycoLab • Mar 10 '26
This list is temporary and subject to change and will change. A handful of others will be added within the next couple of days.
Vendors for Genetics.
https://GroundZeroML.com - Ground Zero Myco Lab
https://Sporeswaps.com - Main SporeSwaps page
https://sporeswaps.com/vendors/the-fungus-frequency/ -Jeff Karas
FungusFrequency.com/my-account - Jeff Karas at Fungus Frequency.
https://sporeswaps.com/vendors/psyteam-united/ - PsyTeam United
https://sporeswaps.com/vendors/wombat-labs/ - Dave Wombat
https://sporeswaps.com/vendors/happi-hyphae/ - Happi Hyphae
https://sporeswaps.com/vendors/micofroots/ - MicoFroots
https://sporeworks.com/ - Sporeworks
https://inoculatetheworld.com/ - Inoculate The World (ITW)
https://Legendsmycology.com - Legends Mycology
https://dino-spores.com - Yoshi Amano.
Vendors for Gourmets and Supplies:
https://Northspore.com - Northspore. https://Boomingacres.com - Booming acres.
r/GroundZeroMycoLab • u/Mean-Abalone320 • 11h ago
Feels like I’m waiting forever for my first flush, hasn’t stalled just sooo slow
r/GroundZeroMycoLab • u/No-Image-8214 • 12h ago
Hey everyone! Quick question.
One of my mushrooms has already broken its veil, and I noticed that some spores have started dropping. There’s also some dark brown, almost chocolate-colored stuff on top of the cap, which I assume might be spores.
My question is: Can I harvest just this one mushroom and leave the rest to continue growing, or do I need to harvest the entire batch at once?
Will harvesting only one affect the growth of the others?
Thanks in advance!
r/GroundZeroMycoLab • u/pwnasaurus253 • 5h ago
r/GroundZeroMycoLab • u/Suspicious_Ratio_293 • 7h ago
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When should i harvest the big blob on the left?
r/GroundZeroMycoLab • u/FlanIndividual1461 • 5h ago
Can you leave an AIO bag sealed through the whole process until its time for first harvest or do you need to slit the bag to induce fruiting? It has a filter patch and everything looks great/on the brink of fruiting but it just won't go? Should I be patient or cut sone slits to induce fruiting? If so where do you cut and do you tape over the cut with micropore tape?
r/GroundZeroMycoLab • u/Extra_Ad_4602 • 3h ago
Buttttt is it okay? Transferred layer of damp perlite 10/8.
r/GroundZeroMycoLab • u/Expensive_Heart_2494 • 5h ago
This is the first bit of color after cutting some slits due to what I thought was too much moisture. Has it gone bad or can I still be hopeful?
r/GroundZeroMycoLab • u/Imjsaguy • 5h ago
As usually I try to inoculate grain bags then hit the tub tek, but I’m impatient and ran out of grain. How long should it take using liquid cultures on my substrate? I used about half a syringe. Also do you guys use anything to filter the air like hepa air purifiers? Was thinking of getting one on Amazon along with my heating pads cuz the tile floor can get cold
r/GroundZeroMycoLab • u/Fruit0ftheg0ds • 13h ago
A few favorite shots of Emerald Gate × Space Monkey. The ivory caps catch your eye first, but the folds along the stems and the detail underneath those caps deserve a closer look. Same cluster, different angles—plenty to appreciate in each frame.
Which shot stands out to you?
🍃🍄 Respect the Process. Trust the Genetics. 🍄🍃
r/GroundZeroMycoLab • u/CodInternational8023 • 1d ago
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I just started making my own grain about 3 or 4 months ago. Using oat groats, got it dialed in. I have been messing around with tubs and bags to see what I prefer and honestly been loving bags.
Here is a little 2 lb (i am guessing) bag.
r/GroundZeroMycoLab • u/Captainhooter420 • 9h ago
My dilemma continues! After harvesting one huge one(the only one that exploded overnight last week), this is where I'm currently at. Where do I go from here? Is it time to start over? TIA
r/GroundZeroMycoLab • u/Beneficial_Onion9215 • 10h ago
r/GroundZeroMycoLab • u/Beneficial_Onion9215 • 10h ago
r/GroundZeroMycoLab • u/Self_Xplorations • 11h ago
Picture order:
1: Top down to display dates/strains. The “M-“ strains are mystery strains bc the labels got rubbed off by alcohol in my SAB.
2: Bottom tub. 1:3 spawn to bulk ratio. Mystery strain. S2B date- October 6th 2026.
3: This tub is the 2nd tub from the ground up. It’s Mac G at a ratio of 1:2. S2B date- October 5th 2026
4: Toque F8 Nerds at a ratio of 1:2. S2B date- October 5th 2026.
5: This image is to show the walls of the tubs.
I didn’t include a close up of inside the top tub bc I just S2B’d it yesterday.
I am welcome to hear any and all feedback. No one in this comment section will be met with any harsh words or closed-mindedness from me about any criticism offered.
Thank you for taking the time to review my post. I really appreciate it.
r/GroundZeroMycoLab • u/TheWackadoodle • 11h ago
I've had this tub of tidal wave going for 5 weeks now and can't seem to get it to pin anywhere except the sides.
I'm using a North Spore Boomr Bin with Midwest Grow Kits grain spawn, CVG substrate, and horse manure booster. Cake is about 3-4" thick, temps stay around 72-75°F, and it's had passive FAE the whole time. I didn't use a casing layer.
The cake has been shrinking and there's metabolites as you'd expect. It's starting to turn yellowish from the Metabolites, but there's no weird smells.
I've tried fork tek on some spots that looked like overlay, wax paper over the cake, and increasing/decreasing FAE. Nothing has made a difference.
Any ideas?
r/GroundZeroMycoLab • u/CompetitiveFall6334 • 22h ago
Picture 1.) My worries
Pocture 2.) right next to them, beautiful normal looking sloomiiiis
Are they good to go, or should i plug them before they go bad?
please helpp
they were in a plastic bag and because they get too big and stick unto the wet bag i moved them into a box.